Freeze-etching methods and applications in membrane research : proceedings of the International Symposium on Freeze-etching, Kapellendorf Castle, GDR, 23-27 July 1979 by International Symposium on Freeze-etching (1979 Kapellendorf, Germany)

Cover of: Freeze-etching | International Symposium on Freeze-etching (1979 Kapellendorf, Germany)

Published by G. Fischer in Jena .

Written in English

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Subjects:

  • Freeze-etching -- Congresses.,
  • Membranes (Biology) -- Congresses.

Edition Notes

Book details

Statementeditor, Helmut Walter Meyer.
SeriesActa histochemica., 23
ContributionsMeyer, Helmut Walter.
Classifications
LC ClassificationsQH236 .I58 1979
The Physical Object
Pagination294 p. :
Number of Pages294
ID Numbers
Open LibraryOL3887944M
LC Control Number81216422

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Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured face that were originally hidden.

A metal/carbon mix enables the sample to be imaged in a SEM (block-face) or TEM (replica). It is used to investigate Freeze-etching book instance cell organelles. Freeze etching is an electron microscopic preparation technique that was developed and used in the 's's.

It is still in use today although less frequently. It is used to fracture frozen biological specimens and observe in particular the e. The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell.

Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze : $ Freeze-etching definition is - freeze fracture that includes the sublimation of surface ice following fracturing especially to reveal additional detail; broadly: freeze fracture.

Central Microscopy Research Facility 85 Eckstein Medical Research Building Iowa City, IA Randy Nessler. This chapter focuses on the freeze-etching method. This method permits an image of the microstructures of the frozen material to be obtained without chemical side effects, that is, the examination of unaltered, viable, stabilized objects by means of electron microscopy.

Rapid freezing, freeze fracture, and deep etching. New York: Wiley-Liss, © (OCoLC) Online version: Rapid freezing, freeze fracture, and deep etching. New York: Wiley-Liss, © (OCoLC) Material Type: Internet resource: Document Type: Book, Internet Resource: All Authors / Contributors: Nicholas J Severs; David Shotton.

Steere RL. Electron microscopy of structural detail in frozen biological specimens. J Biophysic and BiochemCytol. ; – [PMC free article] Pinto da Silva P, Branton D. Membrane splitting in freeze-etching. Covalently bound Freeze-etching book as a membrane marker. J Cell Biol.

; – [PMC free article] Friend DS, Gilula by: 3. Freeze Fracture and Freeze Etching I. PRINCIPLES OF FREEZE FRACTURE TECHNIQUE The technique of freeze fracture is unique Freeze-etching book electron microscopic (EM) methods in that it gives en face views of the internal organisation of biological membranes, allowing the study of the in-plane distribution of integral proteins spanning the lipid bilayer and of other membrane.

Define freeze-etching. freeze-etching synonyms, freeze-etching pronunciation, freeze-etching translation, English dictionary definition of freeze-etching.

A method of specimen preparation for electron microscopy in which a replica is made from a sample that has been rapidly frozen and then fractured along.

ISBN: X X OCLC Number: Description: 55 pages illustrations 25 cm: Contents: Materials and Methods The Nuclear Envelope in Freeze-Etching (Advances in Anatomy, Embryology and Cell Biology) Softcover reprint of the original 1st ed.

Edition by J. Kartenbeck (Author), H. Zentgraf (Author), U. Scheer (Author), W. Franke (Author) & 1 moreAuthor: J.

Kartenbeck. T1 - Freeze fracture and freeze etching. AU - Chandler, Douglas E. AU - Sharp, William P. PY - Y1 - N2 - Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane : Douglas E.

Chandler, William P. Sharp. freeze-etching: [ frēz´-ech″ing ] a method used to study unfixed cells by electron microscopy, in which the object to be studied is placed in 20 per cent glycerol, frozen at −°C, and then mounted on a chilled holder.

Freeze fracture electron microscopy (EM), developed in the s, is an unusual technique that has been used to investigate membrane structure from the perspective of the membrane hydrophobic interior [20].From this technique, the distribution of membrane integral proteins in the lipid milieu can be directly estimated.

Freeze fracture EM is based on rapidly freezing a. If the added freeze-etching step is to be performed, position the cooled microtome arm over the fractured surfaces for one to several minutes.

NOTE: This maneuver will sublime (i.e., etch) water from the fractured surface. The effect of this maneuver is to reveal true cell surfaces, extracellular matrix, and/or molecular assemblies by Cited by: 3. The Nuclear Envelope in Freeze-Etching.

by U. Scheer,W. Franke,J. Kartenbeck,H. Zentgraf. Advances in Anatomy, Embryology and Cell Biology (Book 45/1) Thanks for Sharing. You submitted the following rating and review. We'll publish them Brand: Springer Berlin Heidelberg. The next 15 years saw a series of variations, refinements, and improvements to freeze etching that paralleled a dramatic growth in electron microscope technology.

Throughout the 's, and well into the 's, Erbe and Steere co-authored 34 publications on electron microscopy, especially as it involved freeze etching if cellular tissue.

The purpose of this book is to in a new era, in which cell structures could be provide a bridge, to help those who are familiar visualized down to a macromolecular level.

It is of with conventional electron microscopy, to cross course well recognized that some cell components into the less familiar freeze-etching. Define freeze-etch. freeze-etch synonyms, freeze-etch pronunciation, freeze-etch translation, English dictionary definition of freeze-etch.

A method of specimen preparation for electron microscopy in which a replica is made from a sample that has been rapidly frozen and then fractured along. PDF | On Nov 1,D.S. Branton and others published Freeze-Etching Nomenclature | Find, read and cite all the research you need on ResearchGate.

Shotton DM () Freeze fracture and freeze etching. In: Celis JE (ed) Cell biology: a laboratory handbook, vol 3. Academic, New York, pp – Google ScholarAuthor: Douglas E. Chandler, William P. Sharp.

Freeze-substitution and freeze-drying; Freezing technology; The freeze-etching technique; Freeze-etching methodology; Intepretation of freeze-etching images; Negative staining; Equipment requirements; Negative staining; Summary comments on negative staining procedures; Photography of negatively stained specimens; Particle dimensions and molecular.

An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up totimes shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.

A scanning transmission electron microscope. Methods Mol Biol. ; doi: /_6. Freeze fracture and freeze etching. Chandler DE(1), Sharp WP. Author information: (1)School of Life Sciences, Arizona State University, Tempe, AZ, USA.

Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus Author: Douglas E. Chandler, William P. Sharp. ADVERTISEMENTS: The fluid-mosaic model of membrane structure is beautifully supported by visual evidence provided when freeze-fractured membranes are examined with the transmission electron microscope.

Branton, who pioneered this field, showed that membranes rap­idly frozen at the temperature of liquid nitrogen and cut or chipped with a microtome blade readily frac­ture.

The development of the electron microscope brought one to the other. The purpose of this book is to in a new era, in which cell structures could be provide a bridge, to help those who are familiar visualized down to a macromolecular level.

It is of with conventional electron microscopy, to cross. Freeze Fracture and Freeze Etching. and William P. Sharp 1 (1) School of Life Sciences, Arizona State University, Tempe, AZ, USA. Abstract. Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior.

These large. Replica-Shadowing-And-Freeze-Etching-Sg Adobe Acrobat Reader DCDownload Adobe Acrobat Reader DC Ebook PDF:With Acrobat Reader DC you can do more than just open and view PDF files Its easy to add annotations to documents using a complete set of commenting. The Nuclear Envelope in Freeze-Etching.

Authors: Kartenbeck, J., Zentgraf, H., Scheer, U., Franke, W.W. Free Preview. Identification of chloroplast coupling factor particles, by the freeze-etching and negative-staining techniques, was made utilizing chloroplast thylakoids isolated from spinach leaves.

Surface ice is removed by a vacuum (freeze etching) 4. A thin layer of carbon is evaporated vertically onto the surface to produce a carbon replica. Surface is shadowed with a platinum vapor. Organic material is digested away by acid, leaving a replica.

The anatomy of the cell envelope of a marine vibrio examined by freeze-etching / By. Crawford, Jack Thornton, Type. Book Material. Published material. Publication info. Notes: Typescript. Vita. Thesis (Ph. D.)--University of Florida, Subjects. Brit.J. vener. Dis. () 51, 83 Scanning electron microscopy and.

freeze-etching of gonorrhoealurethral exudate HOMAYOON FARZADEGAN* AND IVAN L. ROTH Department ofMicrobiology, University ofGeorgia, Athens, GeorgiaU.S.A. The pathology of gonorrhoea and the mechanisms by which Neisseria gonorrhoeae establishes diseaseCited by: Looking for abbreviations of FFDE.

It is Freeze-Fracture Deep-Etching Replica. Freeze-Fracture Deep-Etching Replica listed as FFDE. Freeze-Fracture Deep-Etching Replica - How is Freeze-Fracture Deep-Etching Replica abbreviated. freeze-etching; freeze-etching; Freeze-fracture; Freeze-fracture; Freeze-fracture; Freeze-fracture; Freeze.

Rapid Freezing of Biological Specimens for Freeze Fracture and Deep Etching I. PRINCIPLES OF RAPID FREEZING Stabilization of biological structure by the physical process of freezing (cryofixation) forms the starting point for freeze fracture and deep etching (see article by Shotton) and for freeze substitution, cryoultramicrotomy, and cryoelectron microscopy (see articles by.

6/1/09) Note: InOnly a Theory was named a Finalist for Best Science Book of in the Los Angeles Times Book Festival, and was also named a finalist by the National Academy of Sciences for Best Science Book of Print book. Print this chapter. Applications and skills.

Nature of science: Models of membrane structure. The development of the fluid mosaic model of plasma membrane structure is an example of many concepts related to the nature of science, including. Prescott's Microbiology (8th Edition) Edit edition. Problem 6Q from Chapter How are freeze etching and electron cryotomography similar.

Get solutions. Freeze-etching is a method of preparing membranes to give a three-dimensional view of the surface and detail of the membrane’s structures.

Cells are rapidly frozen and fractured by breaking them.John E. Heuser (born Aug ) is an American Professor of Biophysics in the department of Cell Biology and Physiology at the Washington University School of Medicine as well as a Professor at the Institute for Integrated Cell-Matieral Sciences (iCeMS) at Kyoto University.

Heuser created quick-freeze deep-etch electron microscopy (a variant of cell unroofing), a Awards: Member, National Academy .Application Note for Quick Freeze/Deep Etch An ultrastructural imaging technique for biological samples, self-assembled collagen and other biomimetic gels or any hydrated polymeric system.

Introduction. Hydrated polymers are used in many biological and non-biological applications. Example materials include polyvinyl alcohol, collagen, and starch.

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